FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC56445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC56445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6307638
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG35600.026.4748295
CTATACT59950.025.7590714
CCTATAC50100.023.8773423
GTATAGG49550.023.2672421
TATACTG44500.021.6686275
CCCTATA49200.020.0407332
CTTACAC24100.018.651533
TATAGGG49600.018.5635832
TATACTA90800.018.0452295
GTGTAGG34450.017.4694941
CCTACAC32600.017.1243043
TACTAAC52000.017.0079337
CTACACT38850.016.7954354
TACACAG32400.016.7824755
CTACACC46350.016.4240154
CTACCCT31550.016.3154414
TACACCG32000.016.3125675
GTATAAG36900.016.3095931
CTAACCA58100.016.0968029
TATACAG43450.016.0184785
Produced by FastQC (version 0.11.5)