FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC49051.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC49051.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29819818
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG212350.019.6674441
TATACTG233750.019.6334695
CTATACT285200.019.243824
TACACTG176450.017.6681775
CCTATAC207450.017.6491133
TAGACTG136950.016.5701795
GTGTAGG162600.016.3652731
TACACAG173300.016.1485585
GTATTGG241550.016.0292031
GGACCGT99650.014.9877216
CCTACAC161600.014.895043
CTACACT183000.014.7775584
TATACCG131700.014.7534645
ATACTGT260600.014.7450796
GTCTAGG117500.014.4396171
TACACCG140100.014.3863845
TATACAG233250.014.3603595
AGTACTG225300.014.2556655
CTACACC216300.014.2454894
ACTGTGC178750.014.0730528
Produced by FastQC (version 0.11.5)