FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC44480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC44480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8171901
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG69950.023.7370591
CTATACT90400.021.0924324
TATACTG65300.020.6509065
TATAGGG68450.019.5946542
CCTATAC70800.019.3538723
TACACTG49200.017.8303385
TAGACTG35050.017.5820795
GTATAAG52500.016.8492161
TACACCG39200.016.8304085
TGTACTG73050.016.7728085
GTGTAAG35350.016.408911
GTACAGG38600.016.3422221
TATACTA135950.016.1052325
CCCTATA66350.015.9533272
GGACCGT31700.015.7808046
TATACCG35850.015.7741065
TTATACT101650.015.7624684
TACACAG46800.015.6464155
ACTGTGC48150.015.5077348
TACTGTG52050.015.4599117
Produced by FastQC (version 0.11.5)