FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC44442.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC44442.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4850847
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG36200.022.432261
TATAGGG31250.020.4150982
CCTATAC35150.020.0062273
CTATACT38650.019.3199734
CCCTATA29900.018.4272792
GTCCTAG18300.017.432721
GTATTGG36650.017.0132791
TATACTG32850.016.7724695
AGGACAG27550.016.5782155
TTATACT49500.016.5497724
GTATAAG26250.016.2961981
GTGTAGG28050.016.2843821
TAGACTG17950.016.1552755
TATACCG21550.016.1477785
AGTACCG21950.015.8535145
TACACAG24350.015.779595
ACAGTCC17000.015.7783898
CTAGAAC27700.015.4415563
GTATTAT45800.015.3557081
TAGGGGT36600.015.2520584
Produced by FastQC (version 0.11.5)