FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC37108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC37108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18366132
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG147150.023.4545041
CTATACT184550.021.5673944
TATACTG141450.021.4757965
CCTATAC144250.020.8579523
TACACTG105250.018.1852825
GTCTAGG69700.017.6846221
GTGTAGG102150.017.6742341
TATAGGG146200.017.2572362
GGACCGT69350.017.1449266
CCCTATA144150.016.9997062
ACTGTGC110150.016.980248
TAGACAG83850.016.946945
CTACACT113100.016.4103094
TATACAG138400.016.2915555
TAGACTG80350.016.2414515
ATACTGT155550.015.9868296
TCTATAC135750.015.5948943
GTATTGG143250.015.4883941
CTACACC132500.015.4849554
GTCCTAG89650.015.4476591
Produced by FastQC (version 0.11.5)