FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC3401.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC3401.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25742242
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG184750.017.580785
CTATACT213800.017.5318364
GTATAGG149900.017.316351
TACACCG126900.016.911215
CCTATAC143900.016.3744663
TACACTG139150.015.995575
GTGTAGG130000.015.5051641
TATACAG184400.015.2551115
TACACAG141250.015.19315055
CTACACT138050.015.0201484
TATACCG112200.014.9913145
TAGACTG102750.014.8883025
GTCTAGG82550.014.6681161
TCTATAC191400.014.6214333
CTACACC177450.014.4225914
TCTACAC159850.014.3324033
GTATAGA194500.014.2402861
CCAGGAC162250.014.0757143
GTATAAG156000.014.036451
GGACCGT90400.014.0350566
Produced by FastQC (version 0.11.5)