FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC32313.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC32313.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3578978
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG21700.018.0416495
GTCTAGG16700.016.934851
CTTACAC16550.016.6466873
TAGGCTG22550.016.3970555
GTATAAG23450.016.0802841
GTCTTAG14050.015.4838221
CCTGGAC19200.015.481923
TACACAG21650.015.4043065
GTATAGG21700.015.3720331
CCTATAC21900.015.22845653
TTACACT24500.014.7960614
GTATTGG34800.014.79490951
CTGTACT26600.014.4456284
CTAGGCT18100.014.4200294
TAGTACC23250.014.3442254
GTGTAGG20800.014.2939991
CTATACT33650.014.2200474
CCAGGAC19400.014.2011673
GTCTAGT18400.014.1878931
TATACTG29650.014.1822615
Produced by FastQC (version 0.11.5)