FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC32312.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC32312.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25817475
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG150600.016.8015425
TATACTG201250.016.7519655
TACACCG117300.015.8230665
CTATACT231750.015.642274
GTATAGG151700.015.5818031
TACACAG146100.015.4333035
CCTATAC150300.015.2432533
CTACACT155850.014.9795434
TATACAG190600.014.5307875
TAGACTG111100.014.4220465
GTGTAAG106200.014.2694581
GTCTAGG107600.014.0164091
GTCCTGG149100.013.9083011
GTATAAG167550.013.8914091
GTGTAGG141050.013.7253651
GTGTAGA170100.013.683161
GTATAGA216150.013.5187181
TGTACTG231850.013.4777955
GTATTGG220850.013.4280081
GTATTAG182400.013.35672951
Produced by FastQC (version 0.11.5)