FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC32311.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC32311.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7372859
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG58400.017.008425
GTATAGG44150.016.422761
GTCTAGG31800.016.4165651
TAGACAG33350.015.6528315
TACACCG32900.015.6465555
CTACACC48350.015.5952784
TATACAG59050.015.3478085
CCTACAC39350.014.9243883
TACACTG43450.014.8510275
CTATACT68800.014.5427624
CTAGGAC33900.014.1156383
GTCCTGG38550.014.106321
GTCCAGG40800.014.0392461
CCTATAC46050.014.0125313
GTATAAG51950.013.8174051
GTCTTAG33100.013.8003281
TAATACT108400.013.7113694
GTATTAC46450.013.5803331
CTACACT46700.013.5069914
GTGTTAG41800.013.3564591
Produced by FastQC (version 0.11.5)