FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC32281.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC32281.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13697283
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG114600.018.47315
TACACTG81500.017.5246835
GTATAGG81850.017.3625621
GGACCGT43300.016.7438476
CTATACT128650.016.4556924
CCTACAC72050.016.3013743
CTACACT83350.015.9180044
TATACCG58550.015.8498315
CCAGGAC71400.014.9266483
TACACCG56900.014.7804375
TAGACAG63850.014.7612815
CCTATAC81200.014.7323163
CTAGACT70450.014.3046424
CTGTACT97450.014.2100194
GTATAAG95750.014.1605221
TAGACTG58050.014.1129495
CCTGTAC60700.013.9746283
TGTACTG122950.013.9163395
GTCCAGG77450.013.8553261
TATACAG105050.013.8030635
Produced by FastQC (version 0.11.5)