FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC26633.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC26633.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21147164
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG170150.019.5151045
TACACTG125800.019.248785
CCTATAC127600.017.4431553
GTATAGG133600.016.8783251
TACACCG109350.016.7740965
CTATACT194750.016.7149944
CTACACT131550.016.698954
TACACAG121600.016.4555665
TATACCG92850.016.2412245
TAGACTG95250.015.8319975
GTGTAGG119050.015.2260061
GTACCGT80400.015.0590566
GTATAGA177700.015.0561621
ATACTGT179600.014.8552116
GTATAAG137050.014.7604811
TTATACT252950.014.64615254
CCTACAC119300.014.6458273
GTCCTAG103300.014.4591331
GTCTAGG91400.014.3584571
CTACACC157550.014.3573254
Produced by FastQC (version 0.11.5)