FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC26622.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC26622.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20056989
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG110650.018.34771
TATACTG160950.017.297265
TACACTG120050.017.0303695
CTATACT175600.016.927644
TACACAG110950.016.728225
CCTATAC116700.015.9039943
CTAGACT103200.015.8066534
CCTACAC105350.015.6905213
TACACCG89000.015.6404165
CTACACT118800.015.3177284
CTACACC137750.015.05264
TAGACTG86600.014.9855345
GTATTGG156200.014.94685651
GTACAGG96200.014.7725681
TATACCG81150.014.7411895
GTGTAGG106950.014.4402211
TATACAG147900.014.2156565
CTTACAC85300.014.0240033
ACTGCGC69950.013.9910268
GTATAAG132250.013.9803961
Produced by FastQC (version 0.11.5)