FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC26615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC26615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5969122
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG50150.017.2031975
TACACTG38000.017.1708625
GTGTAGA42200.016.4936751
CTACACT35900.015.9538614
ATACTGC34400.015.3850046
TATACCG23350.015.2139645
TAGACAG28600.014.9561285
CTAGGAC26100.014.7220593
CGTATAC21350.014.6017113
CTACCCT30750.014.3819544
TACACAG33450.014.3047755
GCTACAC35250.014.1913323
TGTACTG54300.014.1526985
CTGTACT43300.014.0645084
GTATTGG46250.013.9520151
CCTATAC35500.013.887173
GTATAAG41250.013.88550951
ACTGTGC33950.013.879748
TATACAG44600.013.49200255
TAGGACA41950.013.4801854
Produced by FastQC (version 0.11.5)