FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC26602.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC26602.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15048225
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG86200.017.2422545
GTATAGG100600.016.361551
TATACTG118400.015.7372515
CTACACT83350.015.6572094
CTATACT137150.015.5945794
GTGTAGG75900.015.5719271
TATACCG61250.015.1513515
TAGACTG65800.015.0953135
TACACCG66100.014.9171185
CTACCCT73900.014.4218294
TACACAG83800.014.1023365
TAGGACT88650.014.0668594
GTATAGA124550.013.9139581
TAATACT190200.013.7989334
CCAGGAC77950.013.7655343
GTATAAG102750.013.7609781
CCTATAC92750.013.7577123
CTTACAC60850.013.7020243
CCTACAC71250.013.4318723
GTGTAAG63500.013.3600671
Produced by FastQC (version 0.11.5)