FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC26596.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC26596.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20113562
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG141450.020.7088571
CCTATAC140200.019.7545973
TATACTG159300.018.66045
CTATACT197000.018.5488594
TACACTG124400.016.435455
TATACCG85650.016.4220455
GTATTGG167800.015.5988631
TAGACTG86950.015.5928275
TACACAG116600.015.4207685
CTACACT122450.015.0985154
TATAGGG142250.015.0866312
GTGTAGA142700.014.6334321
GTATAGA167950.014.5056431
TATACTA317650.014.3567015
CCCTATA135100.014.3287662
TATACAG148800.014.1789125
TTATACT262850.014.17777254
GTGTAGG103400.014.1647381
GTATTAC132600.014.1623041
GGACCGT69250.014.1340936
Produced by FastQC (version 0.11.5)