FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC26595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC26595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40137474
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG295050.019.4867571
CCTATAC284650.018.847273
TATACTG320550.018.3422665
CTATACT392300.018.240084
TACACTG240250.017.1099035
CTACACT244100.016.6618424
TAGACTG183050.016.4363845
TACACAG229800.015.8057685
GTATTGG338550.015.7622051
GGACCGT131500.015.3266786
TAGACAG185600.014.8434055
GTGTAGG212350.014.8183371
TATACAG311950.014.734395
CCTACAC215700.014.5870513
ACTGTGC233300.014.5110978
TATACCG175050.014.4955215
GTATAGA327800.014.3105831
TATAGGG291800.014.0872322
CTACACC282250.014.0244754
CCCTATA274200.013.9602872
Produced by FastQC (version 0.11.5)