FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC26594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC26594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6707503
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG43950.017.4873661
CCTATAC40950.016.6422673
TACACCG29350.016.3032575
TACACTG38450.016.2158855
CTATACT59100.016.070244
TATACTG48850.015.7318525
TACACAG36300.015.3788075
GTACCGT22850.015.2297786
CCAGGAC38100.015.2231173
GTATTGG52200.015.0013611
GTGTTAG34200.014.8405281
AGTACCG24650.014.70595
TAGACTG27900.014.551995
ACTGTGC36600.014.261258
TATACAG45800.013.9301485
GTGTATG51100.013.9053711
CTTACAC26600.013.90039353
ACTGCTC36050.013.8755448
GTATAAG44500.013.849571
GTGTAGG32800.013.7055121
Produced by FastQC (version 0.11.5)