FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC26576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC26576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16271135
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG131750.017.5542585
CCTATAC101600.016.7694153
CTATACT159400.016.3741194
TACACTG94600.016.247525
GGACCGT59000.016.0976726
GTATAGG104200.016.0741021
TATACCG65000.016.06175
TAGACTG69850.015.9844145
GTATTGG125550.015.4774891
TAGACAG74100.015.2633115
TACACAG100100.015.2099445
ATACTGT137550.014.7057566
TACACCG74350.014.4318885
CTACACT98650.014.1841714
TATACAG131400.014.0697775
CCTACAC86100.014.0623243
GTATTAC108800.014.0616411
GTGTAAG72500.014.0014321
ACAGTCC55250.013.7772778
GTGTAGG82650.013.7733391
Produced by FastQC (version 0.11.5)