FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_IRGC19928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_IRGC19928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5716483
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG43100.020.3568731
CTATACT54400.019.4582464
CCTATAC44100.018.7420043
TATACTG39150.018.7042085
GTATTGG63200.017.7835121
TACACTG31550.017.4648155
TACACAG29850.017.0021345
TATACCG24500.016.8678025
GTGTAGG29500.016.4685631
CTGTACT37500.015.8537624
GTGTTAC24900.015.7252431
TGCGTTC51400.015.6555319
CCCTATA43450.015.5182562
AGTACTG38900.015.469575
TATAGGG42400.015.3895662
GTATAGT38750.014.969981
TACACCG28600.014.9566975
TATTGGG69400.014.9391652
CTTACAC21900.014.8976623
GTATAAG35350.014.7688271
Produced by FastQC (version 0.11.5)