FastQCFastQC Report
Sat 3 Oct 2020
HFHL7BGXG_n01_GH4-1_Jae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHL7BGXG_n01_GH4-1_Jae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10214222
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAC72900.021.879446
CTAGTAC86100.021.1354583
TAGTACT136950.020.0110954
TATACTG42350.018.6601095
AGTACTA133800.018.423155
ACTACTC88400.018.3695538
TAGACTG31200.017.8927545
TACTACT94450.017.4998197
CCTAGTA98100.017.29372
GTACTAG93850.017.2283441
TATACAG47300.016.8605845
TATACCG21950.016.8452225
GTATAGA54650.015.7880951
CTACTCT97650.015.7392279
GTACAGG28150.015.7117431
TAGGGGT56950.015.1493614
TAGGGCG49800.014.9950865
TACTAGG100350.014.8107682
TACACTG42750.014.754495
GTATTAG50800.014.7009861
Produced by FastQC (version 0.11.5)