FastQCFastQC Report
Thu 11 Mar 2021
HFHKTBGXG_n01_DJ83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHKTBGXG_n01_DJ83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10134276
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT348321934.37067433332189TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTAT765390.7552488209320527TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTGT468320.4621149058896758TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGGAT281120.2773952475736796TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTTT263040.259554801941451TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCGCGTGT187330.18484793585649334TruSeq Adapter, Index 20 (97% over 45bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATATCGTAT145550.14362150784131003TruSeq Adapter, Index 20 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAA121470.11986056033997891TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTAGTAT101700.10035250668128635TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)