FastQCFastQC Report
Thu 11 Mar 2021
HFHKTBGXG_n01_AKM184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHKTBGXG_n01_AKM184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15770325
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC14237589.028082807424704TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATATCGTATGC235090.1490711193333048TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)