FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4400294
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG25350.057.298946
GTATAGG25450.057.0737956
GATAGGT29900.057.0058447
GGATAGT31550.056.9107676
CTATAGG17350.056.485246
CGCGATA8800.056.079994
GCGATAG13800.055.7975465
GGTATAG24850.055.7750935
CGGGATA12800.055.5082554
TATAGGG30250.055.189277
CTGATAG22400.055.1566855
GCGATAA15250.054.6233865
CAGATAG16900.054.4678885
CCGCTAC3350.054.328795
GGCGATA14400.054.201824
CGGATAG11900.054.1180765
GGGATAG28250.054.0181275
CGATAGT16600.053.9769486
AGGATAG18900.053.7041365
TATAGGT37650.053.637987
Produced by FastQC (version 0.11.5)