FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893557
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10000.057.051595
GGATAGT23700.056.2683686
CCCGTCC3000.055.99962610
CGCGATA5500.055.3651854
CTATAGG12800.054.963426
GGATAGG22300.054.935226
GGGATAG21550.054.8971255
CGGGATA10400.053.8476564
GATAGGT22250.053.639167
GCGATAG12150.052.4294435
CGTATAG9550.052.409845
TATAGGC14400.052.2556957
GTATAGG20200.052.1558236
CAGATAG13300.052.106725
TATAGGG25200.051.8043147
GCGTATA10350.051.40244
GCGGATA12700.051.261274
CCCGATA4650.051.1842234
GATAGGG23400.051.1526227
CCTATAG5550.051.0825045
Produced by FastQC (version 0.11.5)