FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778455
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG11250.056.9346055
GGTATAG17800.056.630485
CGCGATA7150.056.2949684
CCTATAG8400.055.8345835
CGTATAG9350.055.776655
CCCGATA4700.055.1076164
GATAGTC12100.054.667697
GGATAGG18850.054.5900846
CGATAGT10850.054.517356
CCCGTCC8650.054.2179410
CGGGATA10550.054.0770384
CTATAGG13400.054.0683756
GTATAGG17350.053.862886
GGATAGT18000.053.8623166
GCGATAA11350.053.657595
CGTATAC3200.053.5949485
TGATAGG22650.052.8488666
CAGATAG13450.052.826465
TATAGCG11250.052.576127
GATAGGG18850.052.544767
Produced by FastQC (version 0.11.5)