FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPHK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPHK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943862
Sequences flagged as poor quality0
Sequence length76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACAC353.7161954E-969.9985632
TCCCCCT700.065.003681
CCACAGC600.064.1653516
AGTCTAC2100.063.3369185
GGCTACC1550.063.2293856
GGGCTAC2050.063.1743055
CCTCTAC1500.063.0035675
GCCTACC501.4551915E-1163.0019548
CTACCCT501.4551915E-1162.9987038
CTGATAC2900.062.7621735
TAACCTG1450.062.758949
CGCTACA1400.062.5035326
CCGCTAC1300.061.926585
TCTACGC1300.061.92187
GTCTACC2150.061.8639646
ACGTTAC850.061.76343523
GCGGATA11050.061.451444
TTCCACC401.0679287E-861.24873719
GCTACAC1550.060.9664887
GTCTCCC1500.060.67016
Produced by FastQC (version 0.11.5)