FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPHK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPHK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107589
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG18100.063.042986
CGTATAC5950.062.3571975
TTATAGC26400.062.049696
CGTATAG13700.060.8070685
CAGATAG20400.060.7394455
GTATAGG18000.060.6708076
CGGATAG12600.060.5596925
CCTATAG10000.060.554145
CTGATAG17700.059.9193465
CTATAGT13250.059.702196
CGCGATA6050.059.5908474
GGGATAG17100.059.360785
ATTATAG27900.058.7136885
GTATAGT17750.058.5673756
CCGCTAC3300.058.3373155
TCTAGCG14700.058.3316887
GATAGTC11900.058.2336467
CACTATA17300.058.0675474
TATAGGC20600.057.7653587
TATAAGG19650.057.708297
Produced by FastQC (version 0.11.5)