FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPHK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPHK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1806249
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCCG150.002224280570.006591
TCCCCCG207.930493E-569.998839
TCTACCG3600.064.1655967
GATAGTC5200.063.9412467
CGGATAG9300.062.853645
CGTATAC3400.062.798295
GGATAGC5500.062.367786
GGTATAG13650.062.3118365
GATAGGG14500.062.2748227
GGATAGG16650.062.226366
TATAGGT16900.062.1291437
CGATAGG7450.062.0175486
GCGATAA6550.061.988855
CGGGATA7350.061.908884
CGATAGT6450.061.3219036
CTACCGG1200.061.248988
GTATAGT14700.060.7183236
GGATAGT13500.060.67076
CCTATAG4850.060.622585
CAGATAG7700.060.458565
Produced by FastQC (version 0.11.5)