FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPHK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPHK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139674
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG11300.061.6394545
CAGATAG20250.061.1874545
CTATAGG20600.059.6381386
TTATAGC34900.059.371836
GGATAGG20800.058.8964166
GGATAGC12500.058.522176
GTATAGC18350.058.3672876
GGGATAG20600.058.2788625
CGTATAG14800.057.9413385
TATAGGC26350.057.646417
GTATAGG23200.057.480586
CGATAGT14450.057.406976
GATAGTC14900.057.0798957
TCTAGCG18100.057.043567
CCGATAA12400.057.018245
CCGATAC4100.056.3435555
CGTATAC7600.056.1862955
CTGATAG20050.056.0369955
ACGATAG12750.056.0020755
TATAGTG33900.055.9580767
Produced by FastQC (version 0.11.5)