FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPHK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPHK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2815626
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC2550.064.5140155
GCTACCG1500.062.9985167
CGCTGAT2850.062.6356663
TCCCACC401.0679287E-861.249649
GCTACCC800.061.2485547
CGATAGG8700.061.153416
GTCGATA9200.060.493084
GCGATAA11350.060.444475
GGGATAG13950.060.218985
CGTATAC5000.060.2039265
CGATATG7400.060.0714846
TCTACGG3350.059.5508357
GATAGTC6700.059.5508357
GGATAGG14500.058.900396
GATAGGC6200.058.708297
CCTGATA8050.058.6994824
CGATAGT8300.058.618286
CTGATAG9050.058.40165
GTATAGG19300.058.397596
GCCGATA4850.057.735724
Produced by FastQC (version 0.11.5)