FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_LPHK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_LPHK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2468450
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC2000.061.25455
GATAGTC9500.060.052187
CCCGTCC9450.059.6291810
CTATAGG11150.058.7038656
GGATAGG18050.058.1760186
GCGATAG10050.058.163485
GGTATAG15250.057.8403175
CGTATAG9650.057.3099145
TCCCGTC8250.057.2722979
GGATAGT15200.057.1094636
CGGGATA7400.056.760934
GATAGGG17400.056.5225647
GTATAGG16800.056.4624826
GATAGGA25050.056.027527
GCGTATA11000.056.0041124
GATAGGT15400.055.68147
TGATAGG21500.055.678516
TATAGGT18400.055.5430567
GGGATAG15750.055.1151665
CGGATAG9550.054.9778635
Produced by FastQC (version 0.11.5)