FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPLK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPLK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661995
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT17950.058.1095286
CGGATAG10250.057.368415
CGATAGG9600.056.5139966
CGCGATA7250.056.0024954
CGATAGT9450.055.929476
GGATAGC6200.055.8906366
TGATAGG16650.055.7092326
GATAGGT17350.055.6765757
CAGGATA9150.055.4669534
GCGATAG9850.055.433945
GTATAGG16300.055.1875426
GCCGATA7300.055.1394424
CAGATAG12450.054.5405775
ACGATAG7800.054.2972955
GATAGGG17950.054.2054867
GGTATAG15250.054.1663485
CGGGATA8650.053.8174254
GCGTATA10350.053.7705084
TGGATAG18300.053.7455675
GTGATAG16900.053.6414455
Produced by FastQC (version 0.11.5)