FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPLK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPLK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5527135
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG37800.062.5925186
CTATAGG28000.062.374936
TTATAGC46750.061.315446
GTATAGG37950.060.961726
CGTATAG23000.060.5651445
GATAGGT36250.060.5367627
TATAGGC38700.060.050527
GGGATAG32600.060.0152665
CTGATAG34500.059.9564485
GGATAGC17050.059.3254436
CGGATAG21600.059.1434485
GATAGTC23600.058.8759847
GTATAGT38200.058.821926
CCTATAG17800.058.792065
GCGTATA18350.058.7465254
TCTAGCG25000.058.518877
TGATAGG47400.058.4071046
CGCGATA10250.058.3901754
TATAGGT44500.058.2011267
CAGATAG25750.058.174695
Produced by FastQC (version 0.11.5)