FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPLK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPLK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2328926
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT7900.059.3673026
ACGATAG6350.058.4254155
CGGATAG7950.057.673175
GGATAGT13250.056.528516
CAGATAG11500.056.3045545
GATAGTC8550.056.080877
CGCGATA5600.055.6252024
GCGATAG9250.055.621835
GCGATAA9900.055.5052535
GTATAGG14250.055.2633636
GGTATAG13150.055.095265
CCGTATA3950.054.936914
CCGATAC2550.054.9021645
TATAGCG7150.054.33477
CGATAGG8050.053.913246
GCCGATA5200.053.846354
CGATATG9850.053.655026
CGCTATA7800.052.500194
GTGATAG14750.052.4408725
GATAGGT16200.052.2830167
Produced by FastQC (version 0.11.5)