FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPLK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPLK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5451368
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGC46250.063.041366
CGTATAC7750.062.324345
GGATAGG32000.062.128476
CTATAGG26400.061.518596
CCTATAG17400.061.3523065
TCTAGCG28450.060.4042667
TGATAGG45200.060.324166
CTGATAG33100.060.167875
CTATAGT24200.059.8793756
GTATAGG34000.059.606276
CGATAGT18600.059.4656836
GATAGTC21100.059.2180567
CGTATAG21950.059.1588485
CCGATAA14100.059.079695
GATAGGT28250.058.9734957
TATAGGC36550.058.891987
GATAGGA51400.058.3560757
ATTATAG56050.058.1996845
TATAAGG35800.057.9749077
CTATACG14650.057.8189286
Produced by FastQC (version 0.11.5)