FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPLK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPLK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755167
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATAG17050.058.5068555
CGATAGG8800.058.468376
CGCGATA5900.058.138034
CGGGATA8950.057.488454
GGATAGT18950.057.4430476
GTATAGG16400.056.343836
ATAGGGT13500.055.7390338
GGTATAG16150.055.482215
CGGATAG8750.055.2023165
GATAGGG17650.055.1257937
CGATAGT12900.055.0798346
GATAGGT18600.054.5682227
GATAGCC3400.054.5571567
CTATAGG11050.054.4819266
GCGTATA9100.054.2330474
GCGATAG11250.054.135615
GATAGTC9300.053.8155567
TATAGGG20600.053.517787
GCGATAA9800.053.2165225
GGCTACG3250.052.7714426
Produced by FastQC (version 0.11.5)