FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPLK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPLK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4585989
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC7100.062.116135
CCTATAG15450.061.8481035
GTATAGC21250.061.6034286
GGATAGG25950.061.371436
TTATAGC39250.060.6403166
CTATAGG24550.060.5946
CAGATAG26450.060.4759565
CGATAGT15600.060.131556
TCTAGCG22800.060.0206877
GTATAGG29200.059.6950956
TGATAGG34000.059.606266
CGGATAG16900.059.4411775
ATTATAG45250.059.3292665
CCGCTAC4500.059.1143955
GATAGTC18650.059.1140567
CGTATAG16250.059.0186655
CTGATAG28300.058.625175
GATAGGA39850.058.229667
TTATACG19300.058.2156756
GTCGATA18650.058.180184
Produced by FastQC (version 0.11.5)