FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253562
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG7300.058.0141225
GGATAGT15300.057.647486
GTATAGG12950.056.4868976
GCGCTAC1500.056.000415
GCGATAA6950.055.3960885
CGGGATA7600.055.263564
GCGTATA6950.054.8924874
GGGGTAC2300.054.7830055
CAGATAG11150.054.6192325
CGATAGT7250.054.069366
CGCGATA4600.053.2612534
CGTATAG6100.052.787275
CCGATAA6700.052.7615785
GATAGGT14600.052.4992187
GCGATAG7950.052.390325
GATAGTG18300.052.2123377
CCCGATA3900.052.0516624
CGATAGG7000.051.5003746
ATAGTGG12100.051.1975868
GGGATAG16200.050.9880265
Produced by FastQC (version 0.11.5)