FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296116
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG10000.061.6067165
CGTATAG12200.061.4001355
GGATAGG20100.060.4294746
CTATAGG15600.060.1347546
CGGATAG11700.059.8355875
GTATAGG18850.059.794316
CTGATAG20050.059.1835065
CAGATAG18200.059.0449035
TTATAGC26500.058.7799956
GTATAGC12450.058.761426
CGTATAC5250.058.6730655
TATAGGC20800.058.5569737
CCGCTAC3000.058.3396955
ATTATAG33450.057.6594965
GGATAGC10200.057.6533436
TATAGGG21550.057.3310787
GATAGGT17100.057.3092357
GGGATAG18550.057.1760445
CGATAGT12250.057.1490866
CTATAGT15350.057.009476
Produced by FastQC (version 0.11.5)