FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2430310
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA9000.059.500684
GGATAGT16800.059.1685646
CGGATAG7900.058.9247255
GTATAGG16550.058.793426
CGTATAG8300.058.615135
GGTATAG14600.056.8157235
TATACGG6300.056.666157
GCGATAA10050.056.418565
CGATAGG8450.055.5047346
TGATAGG15400.055.456326
CGCGATA6700.054.8513764
GATAGTC8850.054.5757757
CGATAGT10200.054.560576
CCGTATA5550.054.234854
CAGATAG13200.054.0915265
CGTGATA9200.054.0223584
GATAGGT17050.053.9877787
GCGTATA10350.053.768734
GATAGGG16300.053.6804967
ACGATAG7000.053.5006145
Produced by FastQC (version 0.11.5)