FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822869
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA5500.057.2718544
GGTATAG17950.055.3752065
GGATAGG20250.055.1349646
CGGATAG10100.055.098175
GTATAGG18300.054.3161166
CCCGTCC5750.054.17308810
GCGATAA11200.054.061685
GGATAGT20350.054.0040936
TATAGCG9100.053.8443837
GATAGGG20950.053.458867
CTATAGG11450.052.8812946
CTGATAG15500.052.6121065
GATAGGT21050.052.539847
CGCTCCC1400.052.4992076
GCGATAG10500.052.332545
CAGATAG12050.052.2813645
TGATAGG22250.052.2239276
TATAGGG22050.052.061787
CGTATAG8700.051.8957635
GATAGTC10600.051.8379177
Produced by FastQC (version 0.11.5)