FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523240
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT17400.058.9358986
CGGATAG9950.058.039335
CGCGATA5950.057.6461944
GCGATAG9350.056.1488885
GTATAGG16800.055.8324936
TATACGG5500.055.361717
CCGTATA5800.054.9129684
TGCGATA10650.054.8818054
CGGGATA10000.054.5991824
CAGATAG12800.054.413255
GATAGGG18000.054.0536737
GCGATAA9600.053.592955
CGTATAG7800.053.3966375
GATAGGT17450.053.350587
GGTATAG15950.053.1026545
CGATAGG8700.052.700366
ACGATAG7350.052.3801655
CGATAGT9800.052.142076
CTGATAG10900.051.6964725
TATAGCG8700.051.492467
Produced by FastQC (version 0.11.5)