FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-40_HPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-40_HPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868700
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA9950.056.6338964
GGATAGG18350.056.2677576
GGATAGT20850.056.0678756
CCCGTCC5300.055.47241610
GCGATAG10950.055.297525
CGTATAC2850.055.263875
CGCGATA6300.055.0007134
CGTATAG9150.054.700165
CAGATAG14550.054.3649675
CGGATAG9400.054.3624045
GATAGGT19800.054.0897257
TATAGGG21700.053.7085047
CTATAGG11950.053.599026
GTATAGG18700.053.3429346
GATAGGG19750.053.163397
GATAGTC10800.053.1469847
CGATAGT9850.052.9448476
GGGATAG19400.052.5006835
TGATAGG21850.052.060176
GGTATAG18750.051.5206645
Produced by FastQC (version 0.11.5)