FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-10_LPK-6S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-10_LPK-6S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5699744
Sequences flagged as poor quality0
Sequence length76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAC9050.068.455755
CCTCTAC6200.066.615545
GTCTACC4750.066.318416
CCGCTAC4400.066.0253455
CCGATAC6450.065.6615145
GCGATAC9600.065.62765
GATAGTC18800.065.531067
CGATACA13700.065.148576
TGCTACC3300.064.699536
CGATATG14750.064.544936
TCTACAC5750.064.52097
GGGATAC11300.064.427335
CTGATAA26750.064.245535
GATACTC5700.063.8588187
CTTCTAC7750.063.6799475
TCTCTAC9400.063.6727335
GGATAGT19900.063.6708646
TCGTACC2200.063.6394442
ACGATAC6600.063.6388855
CTGCTAC4850.063.5076685
Produced by FastQC (version 0.11.5)