FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-10_LPK-5S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-10_LPK-5S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2883387
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCTCC150.002225374669.9989815
CGGATAG10100.061.6865435
CGATAGG9100.060.003286
CGCGATA7300.059.9337464
GGATAGT17000.059.9150476
GTATAGG18700.059.5219766
GGATAGG19400.059.17856
GCGATAA11800.058.732035
CGATATG9250.058.2734576
AGGATAG14900.057.5534865
CCGCTAC2100.056.6697655
GATAGGG20150.056.4507877
GATAGGC9150.056.2286837
TGCGATA10350.056.1373674
CCCGATA4700.055.8531534
CCGTATA6800.055.5903174
CGTATAG11650.055.5824435
CAGATAG11500.055.3943375
CCTGATA6700.055.3752064
TATAGGC12600.055.2769667
Produced by FastQC (version 0.11.5)