FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-10_LPK-4S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-10_LPK-4S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366834
Sequences flagged as poor quality0
Sequence length76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAC5650.066.284655
CCGATAC4050.063.9520345
GCGATAA14650.063.550895
GCGATAC5800.063.363475
GATAGTC8450.062.9571657
GGGATAC5900.062.882745
GGATAGT14150.062.828246
CGATAAC6800.062.7955066
CGATATG8700.062.35776
TGATAGG13350.061.6118476
TCTACCG4700.061.0624587
TCTAGCC1900.060.788117
CCTCGAC1500.060.6680075
GCGATAG12850.060.195885
TGGATAG15300.060.1647345
GGATACG3900.060.129536
CGATACG4100.059.757426
CGATACA6950.059.425776
GATATGG13800.059.3464937
GATAGGT17650.059.290457
Produced by FastQC (version 0.11.5)