FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-10_LPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-10_LPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3359408
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG12750.060.1209456
GTATAGG24750.059.538626
CGGATAG14000.059.502385
CCTGATA8900.058.597854
GGTATAG19200.058.3356635
TATAGCG15850.058.297997
GGATAGG29050.058.195966
CGTATAG13200.058.0705035
CTATAGG15250.058.0687646
TGATAGG23600.057.842166
GGATAGT23700.057.7457856
CCGTATA6450.056.9790234
GATAGGG28750.056.975347
CCCTAGG6050.056.69310410
TATAGGG31050.056.587577
GTGATAG23000.056.4587825
CCTATAG8500.056.4140245
GCGATAG12050.056.35085
GATAGGA26100.056.1891487
CGCGATA7800.056.0919884
Produced by FastQC (version 0.11.5)