FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-10_LPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-10_LPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529976
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT9800.062.146636
GCGATAA15400.062.0492255
CCTATAA17050.061.3820275
GTATAGG23650.061.272226
GTGATAG18000.061.2537165
CGGATAG14300.061.1925245
GGATAGG24050.061.1263736
CTATAGG17650.061.0801966
GTCGATA12550.060.8005034
CGTATAC4900.060.717975
CGTATAG14100.060.5710535
GTCTACC3700.060.5442126
CAGATAG14000.060.5036745
TCTATAG16150.060.2513355
CGATAGG10350.060.196896
TATACGG13000.060.0378537
GATAGTC12550.059.9595537
TGATAGG22450.059.8700036
ACGATAG9300.059.842345
GATAGTG18900.059.8142137
Produced by FastQC (version 0.11.5)