FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-10_LPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-10_LPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119402
Sequences flagged as poor quality0
Sequence length76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC3450.067.972445
TTCTACC3400.065.8847966
CGATACC2550.064.51226
CGATATG8250.064.487246
CGATACA6100.064.264686
TTCTACG5250.064.002376
CGATAGG10950.063.929316
CTTCTAC4500.063.779125
GGGCTAC1600.063.4388355
GCGATAA13050.063.2963565
CGTGTAC1000.063.0013245
GATAGGT11400.062.937917
TGGCTAC2950.062.8826835
CGATATT7800.062.8228386
CCGATAA11250.062.5346535
CTGATAA12550.062.1925475
CCTCTAC4000.062.1263055
TCGATAA13100.061.986045
CTCTACA4750.061.8970346
CGATAAA28400.061.7452556
Produced by FastQC (version 0.11.5)