FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912225
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATA7000.062.001454
CGTATAG9400.061.43765
CGGATAG9250.059.4067885
GGATAGG19500.059.0526586
CGATAGG8550.058.1300136
GATAGTC7500.057.8660247
GGATAGT14050.057.794946
CGATAAA32950.057.6796466
CAGATAG10650.057.184435
GATAGGG18450.056.530547
GATAGGT12500.056.279387
GGTATAG18750.056.1879775
GCGATAG8850.056.15955
CCTAGCC252.3913731E-456.0013055
CCTATAG7950.055.9132545
AGGATAG14200.055.7055245
TGATAGG13450.055.689036
CGATAGT7800.055.6423236
CGTATAC4300.055.350135
ATAGTGC5000.055.299398
Produced by FastQC (version 0.11.5)